The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American anopheline taxonomy - a critical review.

Distribution, Fauna and Seasonal Variation of Sandflies, Simultaneous Detection of Nuclear Internal Transcribed Spacer Ribosomal DNA Gene of Leishmania major in Rhombomys opimus and Phlebotomus papatasi, in Natanz District in Central Part of Iran

Background: Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major is increasing in many parts of Iran. This disease originally is a disease found in gerbils. Leishmania parasites are transmitted by sandflies that live and breed in gerbil burrows. Nested PCR amplified Leishmania ITS1-5.8S rRNA gene in both main reservoir host “Rhombomys opimus” and in the “Phlebotomus papatasi” main vec...

Molecular phylogeny of the family Araceae as inferred from the nuclear ribosomal ITS data

The internal transcribed spacer regions of nuclear ribosomal DNA are widely used to infer phylogenetic relationships in plants. In this study, it was obtained the ITS sequences from 24 samples of Araceae in Iran, representing 3 genera: Arum L., Biarum Schott. and Eminium (Blume) Schott. Phylogenetic analyses were conducted by Bayesian inference and maximum Parsimony methods. Cladistic analysis ...

The Phylogeny of Calligonum and Pteropyrum (Polygonaceae) Based on Nuclear Ribosomal DNA ITS and Chloroplast trnL-F Sequences

This study represents phylogenetic analyses of two woody polygonaceous genera Calligonum and Pteropyrum using both chloroplast fragment (trnL-F) and the nuclear ribosomal internal transcribed spacer (nrDNA ITS) sequence data. All inferred phylogenies using parsimony and Bayesian methods showed that Calligonum and Pteropyrum are both monophyletic and closely related taxa. They have no affinity w...

Development of a New Alternative Diagnostic Tool using Real-Time PCR Coupled with High Resolution Melting (HRM) Analysis Targeting the Second Internal Transcribed Spacer (ITS-2) of Nuclear Ribosomal DNA

Molecular Identification of Polychaetes Based on 18s rRNA Gene 103 7. MOLECULAR IDENTIFICATION OF POLYCHAETES BASED ON 18S rRNA GENE

Molecular tools have revolutionized the exploration of biodiversity, especially in organisms for which traditional taxonomy is difficult (Tang et al., 2012). The small subunit (SSU) 18S rRNA gene is one of the most frequently used genes in phylogenetic studies and an important marker for random target PCR in environmental biodiversity screening. 18S rDNA is a nuclear ribosomal gene, commonly re...